Coumarins fromSeseli devenyenseSimonk.: Isolation byLiquid–Liquid Chromatography and Potential AnxiolyticActivity Using an In Vivo Zebrafish Larvae Mode

Abstract :

Different types of anxiety disorders have become the number one mental health issue indeveloped countries. The search for new, safer and effective drug-like molecules among naturallyderived substances faces two difficulties: an efficient method of isolation compounds with a high-purity and high-throughput animal model for activity assay. Thus, the aim of the present studywas to isolate by liquid–liquid chromatography high-purity rare coumarins from the fruits ofSeselidevenyenseSimonk. and evaluate their anxiolytic effect (defined as reversed thimotaxis) using a5-days post-fertilization (dpf)Danio reriolarvae model. Liquid–liquid chromatography enabled theisolation of one simple hydroxycoumarin (devenyol) and four pyranocoumarins (cis-khellactone,d-laserpitin, isolaserpitin and octanoyllomatin). The anxiolytic effect was defined as a decrease inthe time spent in the boundaries of the living space (also described as reversed thigmotaxis). Ourresults show that all isolated courmarins exerted a significant influence on the anxiety behavior(anxiolytic activity) in the zebrafish larvae model. According to our knowledge, this is the first reportof anxiolytic activity of pyranocoumarins and devenyol.

Link to the publication :

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