- Robotic Liquid Handling Systems
- Microplate Stacking Robotics
- COPAS™ BioSorter™ Systems
- Automated addition of zebrafish embryos/larvae to wells
- Automated addition of other small animal models to wells
Zebrafish exhibit a light avoidance response that can pose additional challenges for fluorescence imaging. Standard fluorescence microscopy delivers excitation light through the objective lens. At magnifications greater than approximately 2.5x this will result in non-uniform illumination of the SideView™ well. The zebrafish will tend to move away from the focal point to the dimmer regions of the well. As the objective power is increased, the intensity of the illumination beam is increased and the animals light avoidance response is further aggravated. Even at low magnification when the well is uniformly illuminated, the introduction of light through the side of the well may cause some of the animals to roll onto their sides.
The solution is to flood the SideView™ wells will excitation light from above. PSI has used this approach with great success.
An LED-based illuminator similar to the one depicted below may be ordered on a custom basis.
Brightfield Imaging optimization :
The illumination configuration in the figure below would improve brightfield imaging of the larvae by providing true transillumination of the sample. The illumination source can either be the standard blue LED illuminator under development for fluorescence excitation, or can be any other wavelength LED including white. Images collected using this approach are of the same quality as images collected using a standard microscope condenser.
Illumination apparatus for brightfield imaging of zebrafish larvae in SideView™ arrays. The LED illumination source is positioned adjacent to the well. Approximately 5% of the light is reflected from the top surface of prism through the well. The light that passes through the sample is efficiently reflected by prism toward the microscope objective.