ZebraBox for embryos or Larvae
Long‑term acclimation to near‑future ocean acidifcation has negligible efects on energetic attributes in a juvenile coral reef fsh
Behavioural responses in effect to chemical stress in fish
Pharmaceutical residues are polluting the surface water environments worldwide. Sewage and wastewater treatment, therefore, needs to be improved in order to remove pharmaceutical residues from the effluent. One such treatment improvement is effluent ozonation. Even though ozonation has proven to be very efficient in reducing pharmaceutical parent compound concentrations in wastewater effluents, much remains unclear regarding potentially toxic ozonation by-product (OBP) formation. In this study, we sought to elucidate the aquatic toxicity of ozonated pharmaceuticals in zebrafish (Danio rerio) embryos in a static 144 h post fertilization (hpf) fish embryotoxicity (ZFET) assay. Three pharmaceuticals commonly detected in wastewater effluents, i.e. carbamazepine, diclofenac, and oxazepam, were selected for testing. Toxicity was assessed before and after 1 min ozonation (0.053 mg L-1 peak O3 concentration) and 10 min ozonation (0.147 mg L-1 peak O3 concentration). Chemical analysis showed that carbamazepine and diclofenac were largely removed by ozone (90±11% and 97±3.8%), whereas oxazepam was removed to a lesser extent (19±5.7%). The ZFET assay revealed diverging toxicities. Diclofenac embryotoxicity decreased with increasing ozonation. Oxazepam did not cause embryotoxicity in the ZFET assay either pre- or post ozonation, but larvae swimming activity was affected at 144 hpf. Carbamazepine embryotoxicity, on the other hand, increased with increasing ozonation. Chemical analysis showed the formation of two OBPs (carbamazepine-10,11- epoxide and 10,11-dihydrocarbamazepine), possibly explaining the increased embryotoxicity. The results of this study highlight the importance of new chemical and toxicological knowledge regarding the formation of OBPs in post-ozonated effluents.
Swimming activity in zebrafish larvae exposed to veterinary antiparasitic pharmaceuticals
Ecotoxicological effects, water quality standards and risk assessment for the anti-diabetic metformin
Identifcation of pathways that regulate circadian rhythms using a larval zebrafsh small molecule screen
Phenotypic Characterization of Larval Zebrafish (Danio rerio) with Partial Knockdown of the cacna1a Gene
A multi-endpoint in vivo larval zebrafish (Danio rerio) model for the assessment of integrated cardiovascular function
The mineralocorticoid receptor is essential for stress axis regulation in zebrafish larvae
Nonlinear mixed-modelling discriminates the effect of chemicals and their mixtures on zebrafish behavior
A novel metabolism-based phenotypic drug discovery platform in zebrafish uncovers HDACs 1 and 3 as a potential combined anti-seizure drug target
Evaluation of neuroactive effects of ethanol extract of Schisandra chinensis, Schisandrin, and Schisandrin B and determination of underlying mechanisms by zebrafish behavioral profiling
Behavioral And Physiological Analysis In A Zebrafish Model Of Epilepsy
High-performance counter-current chromatography isolation and initial neuroactivity characterization of furanocoumarin derivatives from Peucedanum alsaticum L (Apiaceae)
DMSO effects larval zebrafish (Danio rerio) behavior, with additive and interaction effects when combined with positive controls
Effects of 17α‑ethinylestradiol on caudal fin regeneration in zebrafish larvae
Drug repositioning in epilepsy reveals novel antiseizure candidates
Embryonic development, locomotor behavior, biochemical, and epigenetic effects of the pharmaceutical drugs paracetamol and ciprofloxacin in larvae and embryos of Danio rerio when exposed to environmental realistic levels of both drugs
Using Zebrafish for Investigating the Molecular Mechanisms of Drug-Induced Cardiotoxicity
A zebrafish model to study small-fiber neuropathy reveals a potential role for GDAP1
NCBINCBI Logo Skip to main content Skip to navigation Resources How To About NCBI Accesskeys Sign in to NCBI PubMed US National Library of Medicine National Institutes of Health Search database Search term Clear input Advanced Help Result Filters Format: Abstract Send to Chemosphere. 2018 Jan 25;197:611-621. doi: 10.1016/j.chemosphere.2018.01.092. [Epub ahead of print] Developmental toxicity induced by PM2.5 through endoplasmic reticulum stress and autophagy pathway in zebrafish embryos.
A Zebrafish Behavior Assay for Assessing Anti- Epileptic Drug Efficacy
Normalization of large-scale behavioural data collected from zebrafish
Towards less hazardous industrial compounds: coupling quantum mechanical computations, biomarker responses and behavioral profiles identify bioactivity of SN2 electrophiles in alternative vertebrate models
Protecting the environment from psychoactive drugs: Problems for regulators illustrated by the possible effects of tramadol on fish behaviour
Biochemical and behavioral responses of zebrafish embryos to magnetic graphene/nickel nanocomposites
Identification of GSK-3 as a Potential Therapeutic Entry Point for Epilepsy
Sleep deprivation caused a memory defects and emotional changes in a rotenone-based zebrafish model of Parkinson’s disease
Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos
Synergistic effects of Pb and repeated heat pulse on developmental neurotoxicity in zebrafish
Varying the exposure period and duration of neuroactive pharmaceuticals and their metabolites modulates effects on the visual motor response in zebrafish (Danio rerio) larvae
Role of Olfactorily Responsive Neurons in the Right Dorsal Habenula - Ventral Interpeduncular Nucleus Pathway in Food- Seeking Behaviors of Larval Zebrafish
2,4-Dichlorophenoxyacetic acid herbicide effects on zebrafish larvae: development, neurotransmission and behavior as sensitive endpoints
Multigenerational consequences of early-life cannabinoid exposure in zebrafish
Subunits of the mechano-electrical transduction channel, Tmc1/2b, require Tmie to localize in zebrafish sensory hair cells
Biallelic VARS variants cause developmental encephalopathy with microcephaly that is recapitulated in vars knockout zebrafish
Developmental protein kinase C hyper-activation results in microcephaly and behavioral abnormalities in zebrafish
Multi-parametric analysis of ciprofloxacin toxicity at ecologically relevant levels: Short- and long-term effects on Daphnia magna
Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in Zebrafish
Automated morphological feature assessment for zebrafish embryo developmental toxicity screens
We report the development and application of a high-throughput, quantitative screen for drugs that alter the behavior of larval zebrafish. We found that the multidimensional nature of observed phenotypes enabled the hierarchical clustering of molecules according to shared behaviors.
Mitigating Human IAPP Amyloidogenesis In Vivo with Chiral Silica Nanoribbons
Anti-Parkinson's disease activity of phenolic acids from Eucommia ulmoides Oliver leaf extracts and its autophagy activation mechanism
Novel neurotoxic peptides from Protopalythoa variabilis virtually interact with voltage-gated sodium channel and display anti-epilepsy and neuroprotective activities in zebrafish
Assessment of the ecotoxicity of the pharmaceuticals bisoprolol, sotalol, and ranitidine using standard and behavioral endpoints
Municipal wastewater effluent exposure disrupts early development, larval behavior, and stress response in zebrafish
Phytoremediation processes of domestic and textile effluents: evaluation of the efficacy and toxicological effects in Lemna minor and Daphnia magna
Knockdown of chondroitin-4-sulfotransferase-1, but not of dermatan-4-sulfotransferase-1, accelerates regeneration of zebrafish after spinal cord injury
Evaluation of neuroactive effects of ethanol extract of Schisandra chinensis, Schisandrin, and Schisandrin B and determination of underlying mechanisms by zebrafish behavioral profiling
Long-term effects of oxytetracycline exposure in zebrafish: A multi-level perspective
The Locus Coeruleus Modulates Intravenous General Anesthesia of Zebrafish via a Cooperative Mechanism
Developmental neurotoxicity and immunotoxicity induced by graphene oxide in zebrafish embryos
The role of humic acids on gemfibrozil toxicity to zebrafish embryos
RGD inhibition of itgb1 ameliorates laminin-a2 deficient zebrafish fibre pathology
Expression of pathogenic SCN9A mutations in the zebrafish: A model to study small fiber neuropathy
Motor Neuron Abnormalities Correlate with Impaired Movement in Zebrafish that Express Mutant Superoxide Dismutase 1
Advancing the zebrafish embryo test for endocrine disruptor screening using micro‐injection: ethinyl estradiol as a case study
Bioassay-guided isolation of anti-seizure principles from Semen Pharbitidis using a zebrafish pentylenetetrazol seizure model
Combined transcriptomic and proteomic analysis reveals a diversity of venom-related and toxin-like peptides expressed in the mat anemone Zoanthus natalensis (Cnidaria, Hexacorallia)
Highlights Co-exposure with n-TiO2 enhanced PCP-induced thyroid endocrine disruption in zebrafish larvae. Thyroglobulin, deiodinase 2 and 3,5,3′-triiodothyronine were sensitive indicators to the co-exposure. Co-exposure with n-TiO2 did not change PCP-induced neurotoxicity. Published 16 March 2020
Published March 29th 2020
Published March 28th 2020
ecological and health risks of GO exposure
Published March 12th 2020
Neurobehavioral tests were performed on a ZebraBox platform (ViewPoint, France) according to our previous methods [20, 21], which can realize automatic tracking and high-throughput screening of larvae. Locomotion, path angle, and two-fish social activity of the same batch of exposed larvae were adopted to evaluate the neurobehavioral effects induced by BDE-209 at 5, 6, and 7 dpf, respectively, with light stimulus. The protocol of light stimulus included an initial 10 min of light adaption, followed by three repeated cycles with 10 min of dark period and 10 min of light period.
Isoliquiritigenin triggers developmental toxicity and oxidative stressemediated apoptosis in zebrafish embryos/larvae via Nrf2-HO1/ JNK-ERK/mitochondrion pathway
2020, Accepted 18 January 2020
Published: 24 January 2020
Accepted 16 March 2020
The vast majority of neurodegenerative disease cannot be attributed to genetic causes alone and as a result, there is significant interest in identifying environmental modifiers of disease risk.
A set of adverse outcome pathways (AOPs) linking inhibition of thyroperoxidase and deiodinase to impaired swim bladder inflation in fish has recently been developed. These AOPs help to establish links between these thyroid hormone (TH) disrupting molecular events and adverse outcomes relevant to aquatic ecological risk assessment.
Highlights • Parental exposure to PFBS disturbed maternal transcript transfer in zebrafish. Toxic actions on maternal transfer of mRNA depended on exposure concentrations. Parental exposure to 10 μg/L PFBS impacted histone-DNA packaging in nucleosome. Parental exposure to 100 μg/L PFBS mainly affected adherens junction transcripts. Development, growth and neurobehaviors of offspring embryos were impaired.
Cadmium exposure induced changes of locomotor activity in zebrafish. The relative abundances of microbial phyla and genera were changed by cadmium. The expression of bdnf and genes involved in serotonin signaling were changed.
Halimide or plinabulin for use in the treatment or prevention of epilepsy.
GBDP is composed of 46 constituents. Content of 12 constituents were different between GBDP and EGb 761. GBDP attenuated neurological deficits in zebrafish and mice PD models. GBDP prevented PD through anti-apoptosis and Akt/Gsk3β signaling pathways. GBDP might be a potential therapeutic agent for PD.
A POP mixture and PFOS increased swimming speed and thigmotaxis of larval zebrafish. Transcriptomic analysis identified upregulation of muscle related genes. IPA showed that the POP mixture and PFOS activate the calcium signaling pathway. Mechanistic work with RyR antagonist lent support to results obtained from IPA.
Marcus Ghosh1 & Jason Rihel1 1. Department of Cell and Developmental Biology, University College London, London WC1E 6BT, UK
Detection of sound and head movement requires mechanoelectrical transduction (MET) channels at tips of hair-cell stereocilia. In vertebrates, the transmembrane channel-like (TMC) proteins TMC1 and TMC2 fulfill critical roles in MET and substantial evidence implicates these TMCs as subunits of the MET channel. To identify developmental and functional roles of this Tmc subfamily in the zebrafish inner ear, we tested the effects of truncating mutations in tmc1, tmc2a, and tmc2b on in vivo mechanosensation at the onset of hearing and balance, before gender differentiation.
Description of the first deletion mutant for the unique fus orthologue in zebrafish. fus −/− have a reduced life span and severe motor phenotype. fus KO induces anatomical defects at motor neurons and NMJ. fus loss of function is associated with a general misregulation of Tau transcripts.
Highlights Tritia reticulata survival relies on the swimming performance of planktotrophic larva. Swimming and histological integrity of related organs assessed after exposure to OA-W Veligers travelled slower, for shorter distances, under end of century OA-W scenarios. Foot secretor epithelium alteration suggests pre and post-settlement motor impairment. Veliger competence is proved to be threatened if OA-W projections are realised.
Toxic effects of PM on development, locomotor behavior and oxidative stress in larval zebrafish were analyzed. PM exposure delayed development, inhibited hatchability and increased heart rate in larval zebrafish. PM exposure showed abnormal free swimming behavior and swimming behavior in response to light-dark transition. PM affects the activities of CAT, GPX, GST and the transcriptional levels of related genes.
Bis(2-ethylhexyl)-2,3,4,5-tetrabromophthalate affects lipid metabolism in zebrafish larvae via DNA methylation modification
The α1-adrenoceptor inhibitor ρ-TIA facilitates net hunting in piscivorous Conus tulipa
Life history and behavior effects of synthetic and natural dyes on Daphnia magna
Ferredoxin 1b deficiency leads to testis disorganization, impaired spermatogenesis and feminization in zebrafish
Effects of antidepressants with different modes of action on early life stages of fish and amphibians
Inherited and De Novo Genetic Risk for Autism Impacts Shared Networks
Neural signatures of sleep in zebrafish
A long noncoding RNA cluster-based genomic locus maintains proper development and visual function
NEUROPROTECTIVE COMPOSITION , PREPARATION PROCESS THEREOF AND MEDICAL USES THEREOF
Impact of co-exposure to titanium dioxide nanoparticles and Pb on zebrafish embryos
Neurodevelopmental toxicity assessments of alkyl phenanthrene and Dechlorane Plus co-exposure in zebrafish
Behavioral effects of citalopram, tramadol, and binary mixture in zebrafish (Danio rerio) larvae
Coexposure to environmental concentrations of cis-bifenthrin and graphene oxide: Adverse effects on the nervous system during metamorphic development of Xenopus laevis
Correspondence Between Behavioral, Physiological, and Anatomical Measurements of Visual Function in Inhibitory Neuron–Ablated Zebrafish
Pyridox(am)ine 5′-phosphate oxidase (PNPO) deficiency in zebrafish results in fatal seizures and metabolic aberrations
Developmental exposure to lead at environmentally relevant concentrations impaired neurobehavior and NMDAR-dependent BDNF signaling in zebrafish larvae
Chemical Pollution Challenges in the Yangtze River Delta
Zebrafish (Danio rerio) embryo-larvae locomotor activity data analysis: Evaluating anxiolytic effects of the antidepressant compound citalopram
The P450 side chain cleavage enzyme Cyp11a2 facilitates steroidogenesis in zebrafish.
Involvement of peroxisome proliferator-activated receptor γ in anticonvulsant activity of α-asaronol against pentylenetetrazole-induced seizures in zebrafish
Flumazenil-insensitive benzodiazepine binding sites in GABAA receptors contribute to benzodiazepine-induced immobility in zebrafish larvae
ABCC9-related Intellectual disability Myopathy Syndrome is a KATP channelopathy with loss-of-function mutations in ABCC9
Linarin improves the dyskinesia recovery in Alzheimer's disease zebrafish by inhibiting the acetylcholinesterase activity
Elmonem, M. A. et al. Cystinosis (ctns) zebrafish mutant shows pronephric glomerular and tubular dysfunction. Sci. Rep. 7, 42583; doi: 10.1038/srep42583 (2017).
OptoGluNAM4.1, a Photoswitchable Allosteric Antagonist for Real-Time Control of mGlu4 Receptor Activity.
Zhang G, Truong L, Tanguay RL, Reif DM (2017) A New Statistical Approach to Characterize Chemical-Elicited Behavioral Effects in High-Throughput Studies Using Zebrafish. PLoS ONE 12(1): e0169408. doi:10.1371/journal.pone.0169408
Zhang, C. et al. Hormetic effect of panaxatriol saponins confers neuroprotection in PC12 cells and zebrafish through PI3K/AKT/mTOR and AMPK/SIRT1/FOXO3 pathways. Sci. Rep. 7, 41082; doi: 10.1038/srep41082 (2017).
Memory traces are believed to be ensembles of cells used to store memories. To visualize memory traces, we created a transgenic line that allows for the comparison between cells activated during encoding and expression of a memory.
Toxicity assessment of zebraﬁsh following exposure to CdTe QDs
Fish are an important model for the pharmacological and toxicological characterization of human pharmaceuticals in drug discovery, drug safety assessment and environmental toxicology. However, do fish respond to pharmaceuticals as humans do? To address this question, we provide a novel quantitative cross-species extrapolation approach (qCSE) ...
Highlights • The zebrafish embryotoxicity test (ZET) was applied using integrated multiple endpoints. • Morphology, motor activity, histopathology, kinetics and gene expression were studied. • A series of anti-epileptic drugs showed specific sensitivity for each endpoint. • This test strategy may contribute to elucidate the mode of action for developmental toxicity in ZET. • This test strategy may contribute to better define the applicability domain of the ZET.
Highlights • Adult rest mutants engage in erratic swimming and display atypical spatial preferences. • rest mutant larvae are hypoactive when compared to sibling controls. • Adult rest mutant males but not females show diminished reproductive success.
Interhemispheric asymmetry of olfactory input dependent neuronal specification in the adult brain
Eriocaulon buergerianum extract protects PC12 cells and neurons in zebrafish against 6-hydroxydopamine-induced damage
Ecotoxicology with the ZebraBox
In this study, we applied structure-based virtual screening techniques to identify natural product or natural product-like inhibitors of iNOS.
The underlying processes of nociception and pain are, despite the rodent models available, still not fully understood. One of the drawbacks of rodent model systems is the difficulty to screen compound libraries for their influence on nociception, thus slowing down the discovery of novel analgesics for clinical use.
The availability of animal models of epileptic seizures provides opportunities to identify novel anticonvulsants for the treatment of people with epilepsy.
Keywords Buprenorphine; Cyclooxygenase-2; Nociception; Pain; Zebrafish larvae
HIGHLIGHTS - The zebrafish embryotoxicity test (ZET) was applied using integrated multiple endpoints - Morphology, motor activity, histopathology, kinetics and gene expression were studied - A series of anti-epileptic drugs showed specific sensitivity for each endpoint - This test strategy may contribute to elucidate the mode of action for developmental toxicity in ZET - This test strategy may contribute to better define the applicability domain of the ZET
8–48 hpf is the sensitive exposure window for neurodevelopmental and behavioral toxicities of TBBPA. TBBPA induced apoptotic cell death and affected motor neuron and muscle fiber development. Zebrafish embryos rapidly absorbed and accumulated TBBPA, and eliminated it quickly. • TBBPA induced ugt genes expression, which increased T4 metabolism and subsequently induced neurobehavior defects.
Low dose TBBPA chronic exposure reduced zebrafish body weight and length. TBBPA exposure induced adult zebrafish hyperactivity with magnitude bigger in males than in females. Low dose TBBPA exposure induced male zebrafish heightened aggression using mirror attack test.
Extensive ethnopharmaceutical documentation is available on plants with demonstrated psychoactivity in humans (for review (Raetsch 2005)). Even though there is a huge need for new molecules in mental health, this reservoir of knowledge remains greatly untapped. One of the main reasons is probably the lack of a good in vivo system allowing testing of psychoactive efficacy of complex mixtures of unknown composition. Ethnobotanical reports on a specific plant often differ on plant species/subspecies/harvest but also on mode of preparation and administration which all generate a number of imponderable parameters. Therefore, it is necessary to establish a system which would be capable of circumventing those problems. Ideally, the proposed system has to be flexible and sensitive enough to allow rapid and systematic screening of a big number of complex mixtures like crude plants extracts. In such a system, first pass screening could be done with minimally processed extracts like preparations made from dried powdered parts of a plant of interest. Subsequently, gradually refined mixtures could be tested the same way to eventually isolate the active compound(s).
Highlights Custom-made economic imaging setup for distraction-free behavioural recording. ImageJ based algorithm for fast and automated tracking of Zebrafish without plugins. Mathematical workflow for extraction of behavioural endpoints. New representation for spatio-temporal nature of choice-based behaviours.
Best, C. et al. Maternal cortisol stimulates neurogenesis and affects larval behaviour in zebrafish. Sci. Rep. 7, 40905; doi: 10.1038/srep40905 (2017).
The link between tobacco use and the development of serious diseases such as certain cancers, heart disease, respiratory diseases and stroke are well established . Despite this, tobacco use continues to be a growing global epidemic and remains the leading cause of preventable death worldwide . More than 37,000 Canadians have been estimated to die prematurely on an annual basis as a consequence of tobacco smoking  and costs to the health care system are estimated at over $4.3 billion annually . As such, tobacco use remains a public health concern in Canada.
Normal and visually-impaired zebrafish larvae have differentiable light-induced locomotor response (LLR), which is composed of visual and non-visual components
As the population grows, so does the drive for urbanization. Urbanization and the resulting anthropogenic activities have an impact on the quality of nearby water resources. Puerto Rico’s population density is one of the highest in the world. We seek to develop a protocol to measure how Puerto Rico´s high level of urban development has impacted the behavior and nervous system function of river fauna. We have started by recording movement trajectory and velocity of freshwater prawn larvae during five consecutive periods of 10-minutes light/dark cycles, using ViewPoint´s Zebrabox.
Highlights • Zebrafish trpm7 mutant larvae exhibit dopamine-responsive behavioral deficits. • In such larvae, dopaminergic neurons express DAT but many lack expression of TH. • trpm7 mutant larvae are hyper-sensitive to a toxin of dopaminergic neurons, MPP+. • We show that SH-SY5Y cells, which are dopaminergic, depend on TRPM7 for survival. • How Trpm7 contributes to function and viability of dopaminergic neurons is unknown.
Highlights • Both asic1 mRNA and protein expressions were detected in the adult zebrafish retina. • Acidosis-induced currents in the isolated retinal ganglion cells (RGCs) were recorded using whole cell patch clamping. • Blockade of ASICs channel significantly reduced the locomotion of larval zebrafish in response to light exposure.
Zebrafish care The Wild Indian Karyotype (WIK) strain of zebrafish was reared in community tanks on a 14-hour light, 10-hour dark cycle. Fish were fed twice a day, once with formulated feed (Dr. Bassleer Biofish Food) and once with Artemia larvae (cysts from Ocean Nutrition Europe). Eggs were collected 20 minutes after natural spawning and rinsed with system water to minimize infections. Embryos were raised at 28 ± 0.5°C in Danieau's medium. All animal experiments were approved by the Institutional Ethical Committee of the Katholieke Universiteit Leuven and executed in strict accordance with the European Council Directive (2010/63/EC).
Cognitive Aging in Zebrafish
Jingjing Han, Cheng Ji, Yichen Guo, Rui Yan, Ting Hong, Yuanyan Dou, Yan An, Shasha Tao, Fenju Qin, Jihua Nie, Chen Ji, Han Wang, Jian Tong, Wei Xiao & Jie Zhang
Maxinne Watchon, Kristy C. Yuan, Nick Mackovski, Adam J. Svahn, Nicholas J. Cole, Claire Goldsbury, Silke Rinkwitz, Thomas S. Becker, Garth A. Nicholson and Angela S. Laird
VU University in Amsterdam uses the Zebrabox technology to investigate the effect of micro-pollutants.
Drug Screening to Treat Early-Onset Eye Diseases: Can Zebrafish Expedite the Discovery?
Hundreds of top universities and companies using the ZebraBox.
Hypocretin/Orexin Overexpression Induces An Insomnia-Like Phenotype in Zebrafish