The mineralocorticoid receptor is essential for stress axis regulation in zebrafish larvae
Ecotoxicological effects, water quality standards and risk assessment for the anti-diabetic metformin
Swimming activity in zebrafish larvae exposed to veterinary antiparasitic pharmaceuticals
Nonlinear mixed-modelling discriminates the effect of chemicals and their mixtures on zebrafish behavior
Behavioural responses in effect to chemical stress in fish
Pharmaceutical residues are polluting the surface water environments worldwide. Sewage and wastewater treatment, therefore, needs to be improved in order to remove pharmaceutical residues from the effluent. One such treatment improvement is effluent ozonation. Even though ozonation has proven to be very efficient in reducing pharmaceutical parent compound concentrations in wastewater effluents, much remains unclear regarding potentially toxic ozonation by-product (OBP) formation. In this study, we sought to elucidate the aquatic toxicity of ozonated pharmaceuticals in zebrafish (Danio rerio) embryos in a static 144 h post fertilization (hpf) fish embryotoxicity (ZFET) assay. Three pharmaceuticals commonly detected in wastewater effluents, i.e. carbamazepine, diclofenac, and oxazepam, were selected for testing. Toxicity was assessed before and after 1 min ozonation (0.053 mg L-1 peak O3 concentration) and 10 min ozonation (0.147 mg L-1 peak O3 concentration). Chemical analysis showed that carbamazepine and diclofenac were largely removed by ozone (90±11% and 97±3.8%), whereas oxazepam was removed to a lesser extent (19±5.7%). The ZFET assay revealed diverging toxicities. Diclofenac embryotoxicity decreased with increasing ozonation. Oxazepam did not cause embryotoxicity in the ZFET assay either pre- or post ozonation, but larvae swimming activity was affected at 144 hpf. Carbamazepine embryotoxicity, on the other hand, increased with increasing ozonation. Chemical analysis showed the formation of two OBPs (carbamazepine-10,11- epoxide and 10,11-dihydrocarbamazepine), possibly explaining the increased embryotoxicity. The results of this study highlight the importance of new chemical and toxicological knowledge regarding the formation of OBPs in post-ozonated effluents.
Zebrafish neurobehavioral phenomics applied as the behavioral warning methods for fingerprinting endocrine disrupting effect by lead exposure at environmentally relevant level
Long‑term acclimation to near‑future ocean acidifcation has negligible efects on energetic attributes in a juvenile coral reef fsh
The maternal transfer and developmental toxicity of chiral contaminants with respect to enantioselectivity have rarely been investigated. Here, the residues and toxicological responses of cis-BF, a typical chiral pesticide, were studied in the progeny of adult zebrafish exposed to cis-BF (0, 20, 100, and 500 ng/L) for 60 days. Cis-BF enantiomers exhibited the equal maternal transfer potentials. GC/MSD analysis showed that parental 1S-cis-BF exposure could disrupt the components of fatty acids in offspring embryos. In transcriptional expression, the whole differentially expressed genes were significantly enriched in GO categories, including the processes related to lipid biosynthesis/metabolism. The perturbations of fatty acids suggested that cis-BF has potential negative impacts on embryos’ development. Furthermore, enantioselective growth inhibition and developmental neurotoxicity in larvae were also observed. The mRNA expressions of neuronal development genes were significantly changed in 1S-exposed offspring, so were the levels of the neurotransmitters and larval locomotion. Our results show that the cis-BF induced the growth inhibition and neurotoxicity in zebrafish larvae, which may be mediated by the development interference in embryos related to the disrupted fatty acid composition. Furthermore, the toxicological response to 1S-cis-BF was greater than that to the 1R-enantiomer in the offspring of exposed adults.
A novel metabolism-based phenotypic drug discovery platform in zebrafish uncovers HDACs 1 and 3 as a potential combined anti-seizure drug target
Effects of 17α‑ethinylestradiol on caudal fin regeneration in zebrafish larvae
High-performance counter-current chromatography isolation and initial neuroactivity characterization of furanocoumarin derivatives from Peucedanum alsaticum L (Apiaceae)
Sub-lethal UV radiation during early life stages alters the behaviour, heart rate and oxidative stress parameters in zebrafish (Danio rerio)
Using Zebrafish for Investigating the Molecular Mechanisms of Drug-Induced Cardiotoxicity
Drug repositioning in epilepsy reveals novel antiseizure candidates
Evaluation of neuroactive effects of ethanol extract of Schisandra chinensis, Schisandrin, and Schisandrin B and determination of underlying mechanisms by zebrafish behavioral profiling
Protecting the environment from psychoactive drugs: Problems for regulators illustrated by the possible effects of tramadol on fish behaviour
Embryonic development, locomotor behavior, biochemical, and epigenetic effects of the pharmaceutical drugs paracetamol and ciprofloxacin in larvae and embryos of Danio rerio when exposed to environmental realistic levels of both drugs
Normalization of large-scale behavioural data collected from zebrafish
A Zebrafish Behavior Assay for Assessing Anti- Epileptic Drug Efficacy
A zebrafish model to study small-fiber neuropathy reveals a potential role for GDAP1
NCBINCBI Logo Skip to main content Skip to navigation Resources How To About NCBI Accesskeys Sign in to NCBI PubMed US National Library of Medicine National Institutes of Health Search database Search term Clear input Advanced Help Result Filters Format: Abstract Send to Chemosphere. 2018 Jan 25;197:611-621. doi: 10.1016/j.chemosphere.2018.01.092. [Epub ahead of print] Developmental toxicity induced by PM2.5 through endoplasmic reticulum stress and autophagy pathway in zebrafish embryos.
Varying the exposure period and duration of neuroactive pharmaceuticals and their metabolites modulates effects on the visual motor response in zebrafish (Danio rerio) larvae
Multigenerational consequences of early-life cannabinoid exposure in zebrafish
Developmental protein kinase C hyper-activation results in microcephaly and behavioral abnormalities in zebrafish
Synergistic effects of Pb and repeated heat pulse on developmental neurotoxicity in zebrafish
Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos
2,4-Dichlorophenoxyacetic acid herbicide effects on zebrafish larvae: development, neurotransmission and behavior as sensitive endpoints
Role of Olfactorily Responsive Neurons in the Right Dorsal Habenula - Ventral Interpeduncular Nucleus Pathway in Food- Seeking Behaviors of Larval Zebrafish
Novel neurotoxic peptides from Protopalythoa variabilis virtually interact with voltage-gated sodium channel and display anti-epilepsy and neuroprotective activities in zebrafish
Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in Zebrafish
We report the development and application of a high-throughput, quantitative screen for drugs that alter the behavior of larval zebrafish. We found that the multidimensional nature of observed phenotypes enabled the hierarchical clustering of molecules according to shared behaviors.
Automated morphological feature assessment for zebrafish embryo developmental toxicity screens
Mitigating Human IAPP Amyloidogenesis In Vivo with Chiral Silica Nanoribbons
Biallelic VARS variants cause developmental encephalopathy with microcephaly that is recapitulated in vars knockout zebrafish
Identification of GSK-3 as a Potential Therapeutic Entry Point for Epilepsy
Subunits of the mechano-electrical transduction channel, Tmc1/2b, require Tmie to localize in zebrafish sensory hair cells
Sleep deprivation caused a memory defects and emotional changes in a rotenone-based zebrafish model of Parkinson’s disease
Evaluation of neuroactive effects of ethanol extract of Schisandra chinensis, Schisandrin, and Schisandrin B and determination of underlying mechanisms by zebrafish behavioral profiling
Knockdown of chondroitin-4-sulfotransferase-1, but not of dermatan-4-sulfotransferase-1, accelerates regeneration of zebrafish after spinal cord injury
Life history and behavior effects of synthetic and natural dyes on Daphnia magna
Ferredoxin 1b deficiency leads to testis disorganization, impaired spermatogenesis and feminization in zebrafish
Eriocaulon buergerianum extract protects PC12 cells and neurons in zebrafish against 6-hydroxydopamine-induced damage
A long noncoding RNA cluster-based genomic locus maintains proper development and visual function
Neural signatures of sleep in zebrafish
As the population grows, so does the drive for urbanization. Urbanization and the resulting anthropogenic activities have an impact on the quality of nearby water resources. Puerto Rico’s population density is one of the highest in the world. We seek to develop a protocol to measure how Puerto Rico´s high level of urban development has impacted the behavior and nervous system function of river fauna. We have started by recording movement trajectory and velocity of freshwater prawn larvae during five consecutive periods of 10-minutes light/dark cycles, using ViewPoint´s Zebrabox.
Ecotoxicology with the ZebraBox
The underlying processes of nociception and pain are, despite the rodent models available, still not fully understood. One of the drawbacks of rodent model systems is the difficulty to screen compound libraries for their influence on nociception, thus slowing down the discovery of novel analgesics for clinical use.
The availability of animal models of epileptic seizures provides opportunities to identify novel anticonvulsants for the treatment of people with epilepsy.
Highlights • The zebrafish embryotoxicity test (ZET) was applied using integrated multiple endpoints. • Morphology, motor activity, histopathology, kinetics and gene expression were studied. • A series of anti-epileptic drugs showed specific sensitivity for each endpoint. • This test strategy may contribute to elucidate the mode of action for developmental toxicity in ZET. • This test strategy may contribute to better define the applicability domain of the ZET.
RGD inhibition of itgb1 ameliorates laminin-a2 deficient zebrafish fibre pathology
Advancing the zebrafish embryo test for endocrine disruptor screening using micro‐injection: ethinyl estradiol as a case study
Behavioural responses in effect to chemical stress in fish: A review
Fish are an important model for the pharmacological and toxicological characterization of human pharmaceuticals in drug discovery, drug safety assessment and environmental toxicology. However, do fish respond to pharmaceuticals as humans do? To address this question, we provide a novel quantitative cross-species extrapolation approach (qCSE) ...
Toxicity assessment of zebraﬁsh following exposure to CdTe QDs
Linarin improves the dyskinesia recovery in Alzheimer's disease zebrafish by inhibiting the acetylcholinesterase activity
Triphenyl phosphate (TPhP) has been shown to cause developmental neurotoxicty. Considering the visual system is a sensitive target, in the present study, we investigated the potential toxicity of TPhP on the visual development and function ACCEPTED MANUSCRIPT 2 in zebrafish larvae. Embryos were exposed to 0, 0.1, 1, 10, and 30 μg/L TPhP from 2 to 144 hours post-fertilization (hpf). The transcription of photoreceptor opsin genes, and histopathological changes in the retina and visual behavior (optokinetic and phototactic responses) were evaluated. TPhP significantly downregulated the transcription of opsin genes (zfrho, opn1sw1, opn1sw2, opn1mw1, opn1mw2, opn1mw3, opn1mw4, opn1lw1 and opn1lw2) in all exposure groups. Histopathological analysis revealed that the areas of the outer nuclear layer (ONL), inner nuclear layer (INL), and inner plexiform layer (IPL) of the retina were significantly reduced in the 10 and 30 μg/L TPhP groups. The number of ganglion cells was reduced significantly in the 30 μg/L group. The optokinetic response (OKR) and phototactic response showed dose-dependent decreases caused by impaired visual function, which was confirmed by unchanged locomotor activity. The results indicated that exposure to environmentally relevant concentrations of TPhP could inhibit the transcription of genes related to visual function and impair retinal development, thus leading to visual impairment in zebrafish larvae.
In this study, we applied structure-based virtual screening techniques to identify natural product or natural product-like inhibitors of iNOS.
Long-term effects of oxytetracycline exposure in zebrafish: A multi-level perspective
Memory traces are believed to be ensembles of cells used to store memories. To visualize memory traces, we created a transgenic line that allows for the comparison between cells activated during encoding and expression of a memory.
The role of humic acids on gemfibrozil toxicity to zebrafish embryos
Bioassay-guided isolation of anti-seizure principles from Semen Pharbitidis using a zebrafish pentylenetetrazol seizure model
Motor Neuron Abnormalities Correlate with Impaired Movement in Zebrafish that Express Mutant Superoxide Dismutase 1
Expression of pathogenic SCN9A mutations in the zebrafish: A model to study small fiber neuropathy
Impact of co-exposure to titanium dioxide nanoparticles and Pb on zebrafish embryos
Neurodevelopmental toxicity assessments of alkyl phenanthrene and Dechlorane Plus co-exposure in zebrafish
Highlights • Zebrafish trpm7 mutant larvae exhibit dopamine-responsive behavioral deficits. • In such larvae, dopaminergic neurons express DAT but many lack expression of TH. • trpm7 mutant larvae are hyper-sensitive to a toxin of dopaminergic neurons, MPP+. • We show that SH-SY5Y cells, which are dopaminergic, depend on TRPM7 for survival. • How Trpm7 contributes to function and viability of dopaminergic neurons is unknown.
The zebrafish-based assay is a widely used animal model system for cardiovascular research. In this study, we investigated the cardiac defects caused by terfenadine and tested the pharmacological response of digoxin to zebrafish with cardiac defects.
NEUROPROTECTIVE COMPOSITION , PREPARATION PROCESS THEREOF AND MEDICAL USES THEREOF
HIGHLIGHTS - The zebrafish embryotoxicity test (ZET) was applied using integrated multiple endpoints - Morphology, motor activity, histopathology, kinetics and gene expression were studied - A series of anti-epileptic drugs showed specific sensitivity for each endpoint - This test strategy may contribute to elucidate the mode of action for developmental toxicity in ZET - This test strategy may contribute to better define the applicability domain of the ZET
Highlights • Adult rest mutants engage in erratic swimming and display atypical spatial preferences. • rest mutant larvae are hypoactive when compared to sibling controls. • Adult rest mutant males but not females show diminished reproductive success.
Developmental neurotoxicity and immunotoxicity induced by graphene oxide in zebrafish embryos
Combined transcriptomic and proteomic analysis reveals a diversity of venom-related and toxin-like peptides expressed in the mat anemone Zoanthus natalensis (Cnidaria, Hexacorallia)
Keywords Buprenorphine; Cyclooxygenase-2; Nociception; Pain; Zebrafish larvae
The Locus Coeruleus Modulates Intravenous General Anesthesia of Zebrafish via a Cooperative Mechanism
OptoGluNAM4.1, a Photoswitchable Allosteric Antagonist for Real-Time Control of mGlu4 Receptor Activity.
The link between tobacco use and the development of serious diseases such as certain cancers, heart disease, respiratory diseases and stroke are well established . Despite this, tobacco use continues to be a growing global epidemic and remains the leading cause of preventable death worldwide . More than 37,000 Canadians have been estimated to die prematurely on an annual basis as a consequence of tobacco smoking  and costs to the health care system are estimated at over $4.3 billion annually . As such, tobacco use remains a public health concern in Canada.
Zhang, C. et al. Hormetic effect of panaxatriol saponins confers neuroprotection in PC12 cells and zebrafish through PI3K/AKT/mTOR and AMPK/SIRT1/FOXO3 pathways. Sci. Rep. 7, 41082; doi: 10.1038/srep41082 (2017).
Zhang G, Truong L, Tanguay RL, Reif DM (2017) A New Statistical Approach to Characterize Chemical-Elicited Behavioral Effects in High-Throughput Studies Using Zebrafish. PLoS ONE 12(1): e0169408. doi:10.1371/journal.pone.0169408
Best, C. et al. Maternal cortisol stimulates neurogenesis and affects larval behaviour in zebrafish. Sci. Rep. 7, 40905; doi: 10.1038/srep40905 (2017).
Highlights Custom-made economic imaging setup for distraction-free behavioural recording. ImageJ based algorithm for fast and automated tracking of Zebrafish without plugins. Mathematical workflow for extraction of behavioural endpoints. New representation for spatio-temporal nature of choice-based behaviours.
Low dose TBBPA chronic exposure reduced zebrafish body weight and length. TBBPA exposure induced adult zebrafish hyperactivity with magnitude bigger in males than in females. Low dose TBBPA exposure induced male zebrafish heightened aggression using mirror attack test.
8–48 hpf is the sensitive exposure window for neurodevelopmental and behavioral toxicities of TBBPA. TBBPA induced apoptotic cell death and affected motor neuron and muscle fiber development. Zebrafish embryos rapidly absorbed and accumulated TBBPA, and eliminated it quickly. • TBBPA induced ugt genes expression, which increased T4 metabolism and subsequently induced neurobehavior defects.
Extensive ethnopharmaceutical documentation is available on plants with demonstrated psychoactivity in humans (for review (Raetsch 2005)). Even though there is a huge need for new molecules in mental health, this reservoir of knowledge remains greatly untapped. One of the main reasons is probably the lack of a good in vivo system allowing testing of psychoactive efficacy of complex mixtures of unknown composition. Ethnobotanical reports on a specific plant often differ on plant species/subspecies/harvest but also on mode of preparation and administration which all generate a number of imponderable parameters. Therefore, it is necessary to establish a system which would be capable of circumventing those problems. Ideally, the proposed system has to be flexible and sensitive enough to allow rapid and systematic screening of a big number of complex mixtures like crude plants extracts. In such a system, first pass screening could be done with minimally processed extracts like preparations made from dried powdered parts of a plant of interest. Subsequently, gradually refined mixtures could be tested the same way to eventually isolate the active compound(s).
Elmonem, M. A. et al. Cystinosis (ctns) zebrafish mutant shows pronephric glomerular and tubular dysfunction. Sci. Rep. 7, 42583; doi: 10.1038/srep42583 (2017).
Maxinne Watchon, Kristy C. Yuan, Nick Mackovski, Adam J. Svahn, Nicholas J. Cole, Claire Goldsbury, Silke Rinkwitz, Thomas S. Becker, Garth A. Nicholson and Angela S. Laird
Jingjing Han, Cheng Ji, Yichen Guo, Rui Yan, Ting Hong, Yuanyan Dou, Yan An, Shasha Tao, Fenju Qin, Jihua Nie, Chen Ji, Han Wang, Jian Tong, Wei Xiao & Jie Zhang
Measuring Larval Zebrafish Behavior: Locomotion, Thigmotaxis, and StartleMeasuring Larval Zebrafish Behavior: Locomotion, Thigmotaxis, and Startle
Zebrafish care The Wild Indian Karyotype (WIK) strain of zebrafish was reared in community tanks on a 14-hour light, 10-hour dark cycle. Fish were fed twice a day, once with formulated feed (Dr. Bassleer Biofish Food) and once with Artemia larvae (cysts from Ocean Nutrition Europe). Eggs were collected 20 minutes after natural spawning and rinsed with system water to minimize infections. Embryos were raised at 28 ± 0.5°C in Danieau's medium. All animal experiments were approved by the Institutional Ethical Committee of the Katholieke Universiteit Leuven and executed in strict accordance with the European Council Directive (2010/63/EC).
Highlights • Both asic1 mRNA and protein expressions were detected in the adult zebrafish retina. • Acidosis-induced currents in the isolated retinal ganglion cells (RGCs) were recorded using whole cell patch clamping. • Blockade of ASICs channel significantly reduced the locomotion of larval zebrafish in response to light exposure.
Genomic and functional conservation of sedative-hypnotic targets in the zebrafish
Cognitive Aging in Zebrafish
Interhemispheric asymmetry of olfactory input dependent neuronal specification in the adult brain
Normal and visually-impaired zebrafish larvae have differentiable light-induced locomotor response (LLR), which is composed of visual and non-visual components
VU University in Amsterdam uses the Zebrabox technology to investigate the effect of micro-pollutants.
Drug Screening to Treat Early-Onset Eye Diseases: Can Zebrafish Expedite the Discovery?
Hundreds of top universities and companies using the ZebraBox.
With recent advances in behavior tracking technology, we were able to develop a new behavioral analysis multi-cell exposure system named “Multi-DaphTrack” with a high-throughput testing capacity for assessing the behavioral response of Daphnia magna
Hypocretin/Orexin Overexpression Induces An Insomnia-Like Phenotype in Zebrafish