VisioBox

OptoKinetic Response - OKR

A complete eye tracking solution: VisioBox 2.0

Optokinetic response tests are commonly performed when screening  visual acuity in disease models of zebrafish and are of critical importance to help finding new treatments for visual deficiencies. Visual system performance is assessed on the basis of the OptoKinetic Response, which involves reflexive slow stimulus following eye movements alternated with rapid resetting saccades.

Tracking of zebrafish oculomotor performance is generally done using standard handmade OKR drums that lack the possibility to automatically change the stimulus during the experiment, thereby producing misleading results because of the user intervention. To this, the VisioBox technology meets the challenges of zebrafish vision science with precise automation, robust data and a steady zebrafish tracking tool.

                      

 

Enjoy a smooth,controllable and flexible tracking process 

Assessing  visual function in zebrafish larvae should be a smooth,controllable and flexible process allowing you to identify the slightest zebrafish visual defect thanks to:  

  • a full control and adaptability of the zebrafish environment and behavioral response

  • tracking of visual acuity and contrast intensity 

  • the opportunity to adapt the environment to the subject’s behavioral response ( color, light intensity) 

  • differentiation of eyes Slow Phase Velocity (SPV) and Fast Phase Velocity (FVP) 

 

We recently released our new  VisioBox for larvae with a flexible screen to improve the contrast, add a more compact camera,and plate holder handlings to ease the daily routine. After years of collaboration we are glad to match all your expectations between accuracy, robustness,user fluidity, and data analysis. The OKR Larvae is versatile enough to be converted as an OMR for adults. 

                

 

From zebrafish Optokinetic response to zebrafish Optomotor Response  

OptoMotor response (OMR) is a reflex behavior observed when an animal naturally follows a rotating grating around it. It has been widely used to assess the visual functions of zebrafish.

However, the standard protocol for larval fish is not yet applicable in adult zebrafish.Rather than monitoring only the eye movement, the swimming direction will be monitored. If the fish responds to a rotating stimulus by swimming equal amounts of time clockwise or counter clockwise, they would be scored as not detecting the stimulus. If they spend most of their time swimming in the same direction as the stimulus, they detect it. The grating size could be adjusted to determine threshold detection.

 

Define a specific environment projected around the Zebrafish using ZebraLab software:

  • Projection of a light source in front of the Zebrafish,

  • Monitoring of the visual response of the fish,

  • Full control and adaptability of the zebrafish' environment (Stripes colors, number of stripes, rotating speed, half mask,…)

  • Adapt the environment to the subject's behavioral responses

  • Neutral density filters available

     

Track several endpoints 

Locomotion outputs:

 Distance traveled into different types of speed ( Small/ Middle/ Large) defined by the user.

 Position (X;Y) and trajectory, Time spent in each Area (Unlimited number of areas),

 Entry count in each area of interest,

 Counting for each state (very small movement, small movement, ambulatory movement

 Time duration in each state ( small/ Middle / Large speed)

 Transition count of the states,

 Distance swum by the animal in each state,

 Full path of the animal throughout the test,

 Clockwise/Counterclockwise rotation count

 Path Angle Histograms:

 Path Angle (change in moving direction between analyzed position)

 Behavior classification with eight different class of angles

 User defined class angle

 Data analysis in real time

 

See Papers using VisioBox

 

  • Safarian, N., Whyte-Fagundes, P., Zoidl, C. et al. Visuomotor deficiency in panx1a knockout zebrafish is linked to dopaminergic signaling. Sci Rep 10, 9538 (2020).
  • Prahatha Venkatraman, Ishara Mills-Henry, Karthik Ramaswamy Padmanabhan, Pete Pascuzzi, Menna Hassan, Jingyi Zhang, Xinlian Zhang, Ping Ma, Chi Pui Pang, John E. Dowling, Mingzhi Zhang, Yuk Fai Leung; Rods Contribute to Visual Behavior in Larval Zebrafish. Invest. Ophthalmol. Vis. Sci. 2020;61(12)

  • Crouzier, Lucie and Richard, Elodie M. and Sourbron, Jo and Lagae, Lieven and Maurice, Tangui and Delprat, Benjamin. Use of Zebrafish Models to Boost Research in Rare Genetic Diseases.International Journal of Molecular Sciences 22-24(2021)