ZebraCube : adult zebrafish behavior analysis

Long-term observation is a possibility by maintaining environmental conditions constant. The Temperature Control Unit deliverable with the ZebraBox system allows to continuous control over experimental conditions. Temperature can be controlled from 20°C to 30°C. The system is equipped with an UV-C water clarifier to avoid algae and bacterial build-up.

Research using a shock paradigm can easily be standardized with ZebraBox. Thanks to the shocker, enjoy the reproducibility of standardizing your work combined with the automation provided by ZebraBox.

It now features the ability to record very fast behaviors such as startle reflex and PPI analysis, revolutionizing the way you observe larvae in micro-well plates. You are now able to check behaviors that happen in few milliseconds !

Camera at 1000 FPS and electronic modules. Option to perform tests such as Zebrafish prepulse inhibition and sensorimotor gating / PPI.

Experimental device supply allowing to test active avoidance on larvae or adult zebrafish, with visual, sound and electric stimuli.

Dimensions of the tank for adult :

Length = 30 cm, Width = 18 cm, Height = 15 cm

• Each tank will have 2 compartments of 15 cm length separated by a removable wall equipped with a mouth of a shape and dimensions to define before manufacturing.

• The 2 compartments will be equipped with stainless steel plates placed at the bottom and at the surface enabling the electric shocks to be delivered.

Dimensions of the tank for larvae :

Length = 129 mm, Width = 86 mm, Height = 28 mm

• The tank is divided into 8 compartments (2 rows of 4), for up to 8 larvae at the same time. One compartment is sized 34*29 mm with two areas of 14 mm wide.

Visual and electric stimuli will be controlled by the ZebraLab software.

The complete test device will be composed of :

• A tank as described above,

• An individual rack resistant to the shock generator,

• A voltage-controllable generator for the electric stimuli,

• An independent visual stimuli system for each compartment.

ZebraLab 3D is a system to automatically score animal behavior in 3 Dimensional space.

Our technology offers live and/or video-based automated scoring of a large number of movement parameters for different animal species such as fish or insects.

ZebraLab 3D is an extension of ZebraLab and is compatible with all your existing ViewPoint setup.

One step data acquisition :

Unlike any other 3D tracking system, ZebraLab 3D is able to process live and instantly the movement trajectory of free swimming fish in tanks or flying insects.

During the experiment, two video cameras, placed at the top and on the side of the tank, analyses the movemen

t of the subject instantaneously. This setup allows getting the best possible 3D rendering. Unlike other systems using stereo-view requiring several steps to analyze the animal’s behavior (1- get a recording, 2- import to statistical software 3- analyze the recorded videos 4-Export data) ZebraLab 3D will provide all the data in one step.

Real 3D movement :

Thanks to the Side and top view setup, the ZebraLab 3D system offers a much more accurate scoring of fish swimming activity, getting rid of any systematic errors or blind spots, especially when the animal dives towards the bottom of the tank or moves towards the walls.

3D Visualization :

ZebraLab creates 3D visualizations and statistics of the swimming path of the fish instantly.

You can display and export the animal’s movements in a 3D visualization as a picture or as a video. Watch it from any angle, zoom it and even replay it step by step. ZebraLab 3D can help you bring alive your talks or presentations.

Extension of the ZebraLab system for Rotation and Histograms including:
 High-Throughput screening detection of up to four arenas simultaneously per camera
 Animal Rotation measurement:
- Body rotation count
- Circling behavior
- Clock wise/Counter clockwise rotation count
 Path Angle Histograms:
- Path Angle (changes of direction of trajectories along the subject’s path)
- Lateral body shifting / Lateral Swerving tendencies
- Behavior classification with eight different class of angles
- User defined class angle

Extension of the ZebraLab system for Social contact and interaction including:
High-Throughput screening detection up to four arenas simultaneously per camera
Social behavior measurement for up to two animals housed in a single arena
- Distance between two animals
- Animal to Animal contacts detection (contact count)
- Time spent in which two animals are within each other’s proximity
- Fusion; sexual behavior copulation

Extension of the ZebraLab system for Group locomotion tracking of different subjects in the same
region, including:
 Group behavior analysis
- Inter-individual distance (Mean distance from each subject to all others)
- Nearest neighbor distance (Mean Distance between the subject and its nearest neighbor)
- Polarization (Magnitude of the mean vector of all subjects)
- Speed Momentary speed for each fish (mean)

Aquatic T-Maze for spatial cognition and learning and memory tests on adult zebrafish compatible
with the ZebraCube, ZebraSquare and ZebraTower with the following specifications:
 3 arms
 Tanks at the end of each arm
 Infrared lighting compatible for dark condition tracking:
 Total size: 50 x28 cm // Height 6 cm
- Start box: 10 x 8.5 cm
- Arm size: 12.5 x 4.5 cm
- Two available colors: White or Transparent
- Single piece structure (no assembly) for robustness and leak proof