SETAC2022 : behavioral ecotoxicology

Published: 04-08-2021 In Case Studie


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Presentation of SETAC EUROPE 2022 

 

  Meet us at SETAC EUROPE  32nd Annual Meeting

  ViewPoint is proud to be an SETAC Europe Partner and to attend the SETAC Annual Meeting 2022 in Copenhagen

 

Review of new behavioral research capabilities for contaminants of emerging concerns.

 

From zebrafish compound screening to water toxicity assessment: new capabilities for behavioral research

Our world is facing the presence of an increasing amount of emerging contaminants of concern and aims to go "towards a reduced pollution society". The use of invertebrates in environmental science shows many advantages to evaluate the effects of chemicals and/or recombinations of substances.

Behavioral ecotoxicological assessment of chemicals and mixtures has long relied on the study of zebrafish among others. Since 2002, evaluating the toxicity of compounds has been possible with the high throughput screening of zebrafish models in microwell plates and Petri dishes.

Our Zebrabox is part of this crucial research for the last 30 years and still counting: Used in several fields such as the measure of the impacts of chemical contaminants, water quality control, or nano plastics pollution, ViewPoint follows his main guideline: develop a tool that matches the research !

 Many scientific papers were published by researchers using our tools. See the latests :

 -  Effects of common environmental endocrine-disrupting chemicals on zebrafish behavior in Water Research  DOI : 10.1016/j.watres.2021.117826 Nature January 2022

This study published in Nature should be interpreted as a positive step in scientific progress as it enlarges the current knowledge held on the effects of endocrine-disrupting chemicals on zebrafish behavior.
By taking a close look at zebrafish locomotor behavior, Chinese identified new disorders induced by contaminants. Neurodegenerative disorders with reduced activity of AChE and oxidative stress are some of the disorders highlighted.

 

Endocrine disrupting chemicals effects on zebrafish behavior

 

 

- Detrimental impact of fine dust on zebrafish: Investigating a protective agent against ocular-damage using in vitro and in vivo models 

DOI : 10.1016/j.chemosphere.2022.133602

Interest in the effects of fine dust on the aquatic environment has lain dormant. Therefore, Korean scientists thus decided to explore the effects of SFD, taking zebrafish as a model organism. Results showed fine dust to cause increased occular angiogenesis in the zebrafish while α/β-adenosine contribute to reduce angiogenesis.

 

Fine Dust and aquatic ecosystems


 -  Road Runoff Characterization: Ecotoxicological Assessment Combined with (Non-)Target Screenings of Micropollutants for the Identification of Relevant Toxicants in the Dissolved Phase 

DOI : 10.3390/w14040511  February 2022

 - Role of endocrine disruption in toxicity of 6-benzylaminopurine (6-BA) to early-life stages of Zebrafish  DOI : 10.1016/j.ecoenv.2022.113287   February 2022

 - Getting more out of zebrafish light dark transition test  

DOI : 10.1016/j.chemosphere.2022.133863 February 2022

 -Cardiovascular toxicity assessment of polyethylene nanoplastics on developing zebrafish embryos  : Exposure to hazardous substances such as nano plastics could cause cardiac disorders. Investigating the effect of nano plastics on zebrafish larvae behavior,scientists found the substances to induce cardiac dysregulations, oxidative stress and systemic inflammation.

DOI :  /10.1016/j.chemosphere.2021.131124

Effects of nano plastics on zebrafish

 -  Environmentally relevant concentrations of microplastics influence the locomotor activity of aquatic biota 

DOI :  10.1016/j.jhazmat.2021.125581 March 2021

 

- Planarian behavioural endpoints in ecotoxicology: A case study evaluating mercury and salinity effects 

DOI : 10.1016/j.etap.2021.103747

The need to embrace diversity in ecotoxicology is central to discover new acute behavioral endpoints. Based on this, scientists found planarians to be relevant test organisms to assess the impacts of contaminants on freshwater organisms. MicroZebralab software helped Brazilians to quantify the locomotion of freshwater invertebrates in their effort to bring to light valuable behavior changes to assess the impact of contaminants.

Evaluating mercury and salinity effects on planarians

 

New Technological advances : 

What if new technological advances could provide researchers with more versatile tools to assess contaminants in multiple small organisms or help evaluate the risk of water pollutants in the water cycle? That is the perspective ViewPoint chose to contribute as ecotoxicological research is on the rise to prevent the harms of climate change on our environment. To support researchers’ efforts and extend our know-how acquired with ZebraBox we assume that the screening capacities could be widened in order to test new types of aquatic species.

 

New approach for screening of Contaminants of emerging concern

 

Tracking a broader range of aquatic species simultaneously

Following on from the technology introduced in 2002, ToxMateLab was developed as the first screening tool of its kind to monitor multi-species behavioral responses simultaneously in a specific environment. Unlike other testing methods which remain limited to the screening of Danio rerio, Medaka, Astyanax Mexicanus, Cave Fish the idea behind ToxMateLab: https://www.viewpoint.fr/en/p/equipment/toxmatelab) was to design a system able to track acute behavioral endpoints in a broad range of aquatic species. This especially opens the way to test other species than zebrafish with the selection of native species to truly understand the response to chemical exposure of a particular population from a local perspective.

Tracking a broader range of aquatic species simultaneouslyIncreased flexibility and scope for screening of neurotoxic chemicals

Behavioral tracking of amphipods, worms, mayfly, tadpoles remains essential to evaluate the impacts of water contaminants to assess the quality of our water. Faced with this challenge, ecotoxicological research capabilities are today largely improved with the opportunity to track up to 3 different species in three independent panels allowing to test animal models under some condition or rather, different ones in order to evaluate the locomotor activity with different scenarios: species/contaminants/concentrations. With such an open environment, compound assessment is facilitated and labs such as the Student's Lab from the Frankfurt Goethe University can benefit from reliable and scalable solutions to track multiple behavior endpoints to assess the impacts of water contaminants. 

 

Check out how the Department of Evolutionary Ecology & Environmental Toxicology (E3T) from Frankfurt Goethe University is upgrading its ecotoxicological research: https://www.bio.uni-frankfurt.de/94291

Student's Lab Goethe goes environment

 Prof. Dr.rer.nat. Henner Hollert from the Goethe Universitat Frankfurt is currently studying the behavioral response of the Gammarus pulex to neurotoxic substances and some of its Ph.D. used ToxMateLab to evaluate the risk characterization of emerging pollutants in the water cycle

 

 The Swiss Ecotox Centre also trusts ToxMateLab for assessing the impacts of chemicals on Gammarus for their ecotoxicological research projects.


 

 

          Zebrabox , Zebralab ,

                                                    ToxmateLab , ZebraCube

 

 

Shifting from toxicological assessment to online biomonitoring of water pollution for wastewater plants and drinking water plants

 

The increase of chemical contaminants including surfactants, biocides, heavy metals, pesticides, and pharmaceuticals is increasing pressure on our aquatic environment and putting human health at risk with endocrine disruptors. Aware of the potential harms, new methods are currently used to detect exposure to contaminants and assess the effects of these pollutants.

 

From this, with the idea that there is no better way to assess water pollution than with the observation of the behavioral response of aquatic biomarkers; ViewPoint recently transferred its multispecies screening tool ToxmateLab to the monitoring of water quality for industrial and municipal wastewater and drinking water plants with (in situ) ToxMate.

 

ToxMate is today the first multi-species water quality biomonitoring station measuring the impact of the presence of chemical pollutants using three different local water species, representative of local aquatic environments. Already deployed in several sites across France and Switzerland to monitor micropollutants in real-time,  Toxmate is a dedicated tool for a major challenge: keep our water clean!

 

If you want to know more, visit https://www.toxmate.fr/en/